Journal: Head and Neck Pathology

Article Title: Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

doi: 10.1007/s12105-019-01114-1

Figure Lengend Snippet: a Photomicrograph depicting grid aided image analysis for calculation of percentage expression of SOX2 and OCT4 upon immunohistochemistry (400X, positive cells tagged red+ , negative cells tagged green+). b–d Photomicrographs showing scoring grades of intensity (b-weak, c-moderate, d-strong)

Article Snippet: Formalin fixed paraffin embedded (FFPE) blocks of each, 4 microm thick sections on poly l lysine coated slides were subjected to immunohistochemical analysis of SOX2 (RTU, Primary antihuman rabbit polyclonal antibody, Master Diagnostica, Spain), OCT4 (RTU, Primary antihuman rabbit monoclonal antibody OCT, Master Diagnostica, Spain) and WNT5A (Primary antihuman rabbit monoclonal antibody, 1:1000, Abexxa, UK).

Techniques: Expressing, Immunohistochemistry

Journal: Head and Neck Pathology

Article Title: Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

doi: 10.1007/s12105-019-01114-1

Figure Lengend Snippet: Showing demographic data and percentage expression of SOX2 and OCT4 in oral squamous cell carcinoma (OSCC), oral epithelial dysplasia (OED) and normal oral mucosa (NOM)

Article Snippet: Formalin fixed paraffin embedded (FFPE) blocks of each, 4 microm thick sections on poly l lysine coated slides were subjected to immunohistochemical analysis of SOX2 (RTU, Primary antihuman rabbit polyclonal antibody, Master Diagnostica, Spain), OCT4 (RTU, Primary antihuman rabbit monoclonal antibody OCT, Master Diagnostica, Spain) and WNT5A (Primary antihuman rabbit monoclonal antibody, 1:1000, Abexxa, UK).

Techniques: Expressing

Journal: Head and Neck Pathology

Article Title: Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

doi: 10.1007/s12105-019-01114-1

Figure Lengend Snippet: SOX2 expression by IHC in NOM,OED and OSCC. a Showing negative expresion of SOX2 in NOM (100X). b Showing positive nuclear expresion of SOX2 in dysplastic cells of OED (400X). c Showing positive nuclear expresion of SOX2 in dysplastic islands of OSCC (400X)

Article Snippet: Formalin fixed paraffin embedded (FFPE) blocks of each, 4 microm thick sections on poly l lysine coated slides were subjected to immunohistochemical analysis of SOX2 (RTU, Primary antihuman rabbit polyclonal antibody, Master Diagnostica, Spain), OCT4 (RTU, Primary antihuman rabbit monoclonal antibody OCT, Master Diagnostica, Spain) and WNT5A (Primary antihuman rabbit monoclonal antibody, 1:1000, Abexxa, UK).

Techniques: Expressing

Journal: Head and Neck Pathology

Article Title: Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

doi: 10.1007/s12105-019-01114-1

Figure Lengend Snippet: Coexpression of SOX2 and OCT4 in OED and OSCC. a Positive co-expression of SOX2 and OCT4 in same location of OSCC tissue specimen (400X) (green arrows). b Positive co-expression of SOX2 and OCT4 in same location of OED tissue specimen (400X) (red arrows)

Article Snippet: Formalin fixed paraffin embedded (FFPE) blocks of each, 4 microm thick sections on poly l lysine coated slides were subjected to immunohistochemical analysis of SOX2 (RTU, Primary antihuman rabbit polyclonal antibody, Master Diagnostica, Spain), OCT4 (RTU, Primary antihuman rabbit monoclonal antibody OCT, Master Diagnostica, Spain) and WNT5A (Primary antihuman rabbit monoclonal antibody, 1:1000, Abexxa, UK).

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Chondrocyte gene network of CSGalNAcT-1 was classified as glycan biosynthesis and metabolism and functional pathway

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Functional Assay

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 mRNA expression in osteoarthritis (OA) cartilage, Kashin-Beck disease (KBD) cartilage and normal cartilage. CSGalNAcT-1 and Hapln-1 mRNA levels reach statistical significance. * Denotes significant difference at P < 0.05

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Comparison of CSGalNAcT-1 expression in the cartilage from osteoarthritis (OA), Kashin-Beck disease (KBD) and normal groups. a , b , c Denote the upper of normal, OA and KBD cartilage, respectively. d , e , f Denote the middle of normal, OA and KBD cartilage, respectively. g , h , i Denote the deep of normal, OA and KBD cartilage, respectively

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 protein level expression reduced in osteoarthritis (OA) and Kashin-Beck disease (KBD) cartilage compared with normal controls. Wnt 3a, β-catenin and Runx-2 level expression increased in OA and KBD cartilage compared with normal controls. A KBD free-Se group. B 0.25 μg/ml Se + KBD group. C 0.10 μg/ml Se + KBD group. D 0.05 μg/ml Se + KBD group

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibitory effect of microRNA-455-5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31

doi: 10.3892/etm.2018.6820

Figure Lengend Snippet: Expression of Rab31 protein in ESCC Eca109 cells. Cells were transfected with miR-NC, miR-455-5p mimics, miR-NC or miR-455-5p inhibitor. Rab31 protein was measured using Western blotting. *P<0.05 vs. miR-NC groups. miR, microRNA; ESCC, esophageal squamous cell carcinoma; NC, negative control.

Article Snippet: Membranes were blocked with 5% skimmed milk at room temperature for 1 h. Subsequently, membranes were incubated with rabbit anti-human Rab31 polyclonal primary antibody (1:1,000; BS70807) and rabbit anti-human GAPDH primary antibody (1:5,000; AP0063; both Bioworld Technology, Inc., St. Louis Park, MN, USA) at 4°C overnight.

Techniques: Expressing, Transfection, Western Blot, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibitory effect of microRNA-455-5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31

doi: 10.3892/etm.2018.6820

Figure Lengend Snippet: Effect of Rab31 expression on proliferation, migration and invasion in ESCC Eca109 cells. (A) Proliferation of Eca109 cells following transfection with miR-NC, miR-455-5p mimics or miR-455-5p mimics + Rab31. *P<0.05 vs. miR-NC group and #P<0.05 vs. miR-455-5p mimics group. (B) Proliferation of Eca109 cells following transfection with miR-NC, miR-455-5p inhibitor or miR-455-5p inhibitor + shR-Rab31. *P<0.05 vs. miR-NC group and &P<0.05 vs. miR-455-5p inhibitor group. Cell proliferation was determined by using a Cell Counting Kit-8 assay. (C) Migration and invasion of Eca109 cells following transfection with miR-NC, miR-455-5p mimics or miR-455-5p mimics + Rab31. *P<0.05 vs. miR-NC group and #P<0.05 vs. miR-455-5p mimics group. (D) Migration and invasion of Eca109 cells following transfection with miR-NC, miR-455-5p inhibitor or miR-455-5p inhibitor + shR-Rab31. *P<0.05 vs. miR-NC group and &P<0.05 vs. miR-455-5p inhibitor group. ESCC, esophageal squamous cell carcinoma; miR, microRNA; NC, negative control.

Article Snippet: Membranes were blocked with 5% skimmed milk at room temperature for 1 h. Subsequently, membranes were incubated with rabbit anti-human Rab31 polyclonal primary antibody (1:1,000; BS70807) and rabbit anti-human GAPDH primary antibody (1:5,000; AP0063; both Bioworld Technology, Inc., St. Louis Park, MN, USA) at 4°C overnight.

Techniques: Expressing, Migration, Transfection, Cell Counting, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibitory effect of microRNA-455-5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31

doi: 10.3892/etm.2018.6820

Figure Lengend Snippet: The investigation between miR-455-5p and Rab31 mRNA was assessed using a dual luciferase reporter assay. Plasmids (0.5 µg) with WT or mutant 3′-untranslated region DNA sequences were co-transfected into Eca109 cells with miR-455-5p mimics. After cultivation for 24 h, ells were lysed using a dual luciferase reporter assay kit and the fluorescence intensity was measured using a GloMax 20/20 luminometer. Renilla was used as internal reference and the fluorescence values of each group of cells were measured. *P<0.05 vs. NC group. miR, microRNA; ESCC, esophageal squamous cell carcinoma; NC, negative control.

Article Snippet: Membranes were blocked with 5% skimmed milk at room temperature for 1 h. Subsequently, membranes were incubated with rabbit anti-human Rab31 polyclonal primary antibody (1:1,000; BS70807) and rabbit anti-human GAPDH primary antibody (1:5,000; AP0063; both Bioworld Technology, Inc., St. Louis Park, MN, USA) at 4°C overnight.

Techniques: Luciferase, Reporter Assay, Mutagenesis, Transfection, Fluorescence, Negative Control